 |
|
Detection of HTLV-III RNA in Lungs of Patients With AIDS and Pulmonary Involvement
Karen J. Chayt, MD;
Mary E. Harper, PhD;
Lisa M. Marselle;
Edward B. Lewin, MD;
Richard M. Rose, MD;
James M. Oleske, MD, MPH;
Leon G. Epstein, MD;
Flossie Wong-Staal, PhD;
Robert C. Gallo, MD
JAMA. 1986;256(17):2356-2359.
Abstract
| |
A majority of pediatric patients and rare adult patients with the acquired immunodeficiency syndrome (AIDS) develop a chronic respiratory disorder referred to as "lymphocytic interstitial pneumonitis" (LIP). Efforts to identify an infectious agent responsible for this process so far have failed. In this study, frozen sections of lungs from patients with AIDS and pulmonary disease were tested by in situ molecular hybridization for the presence of cells infected with human T-cell lymphotropic virus type III (HTLV-III) and expressing viral RNA. In the case of an infant with LIP, a relatively high frequency (0.1 %) of cells in the lung were found to be positive for HTLV-III RNA. This number is the lower limit of total cells infected since the in situ hybridization technique as applied in this study depends on expression of HTLV-III genes, and previous evidence indicates that a proportion of cells infected with HTLV-III may not express viral RNA. Moreover, this degree of infection of the lung is likely limited to LIP, since in ten patients with AIDS and pulmonary diseases other than LIP, only 0% to 0.002% of cells in lung were positive for viral RNA expression. Thus, HTLV-III may play a direct causal role in the development of LIP in infected patients, implicating its involvement in yet another of the diverse clinical diseases associated with this virus.
(JAMA 1986;256:2356-2359)
Author Affiliations
From the Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, Md (Drs Chayt, Harper, Wong-Staal, and Gallo); the Division of Pediatric Infectious Diseases, Henry Ford Hospital, Detroit (Ms Marselle); the Department of Medicine, New England Deaconess Hospital and Harvard Medical School, Boston (Dr Lewin); the Biotech Research Laboratories, Rockville, Md (Dr Rose); and the Departments of Pediatrics (Drs Oleske and Epstein) and Neurology (Dr Epstein), University of Medicine and Dentistry of New Jersey, Newark.
Footnotes
Reprint requests to Laboratory of Tumor Cell Biology, National Cancer Institute, Bethesda, MD 20892 (Dr Gallo).
 CiteULike  Connotea  Del.icio.us  Digg  Reddit  Technorati  Twitter
What's this?
THIS ARTICLE HAS BEEN CITED BY OTHER ARTICLES
|
Lymphoid Interstitial Pneumonia: A Narrative Review
Swigris et al.
Chest 2002;122:2150-2164.
ABSTRACT
| FULL TEXT
Endothelial Cells Enhance Human Immunodeficiency Virus Type 1 Replication in Macrophages through a C/EBP-Dependent Mechanism
Lee et al.
J. Virol. 2001;75:9703-9712.
ABSTRACT
| FULL TEXT
Patterns of Chemokine Receptor Fusion Cofactor Utilization by Human Immunodeficiency Virus Type 1 Variants from the Lungs and Blood
Singh et al.
J. Virol. 1999;73:6680-6690.
ABSTRACT
| FULL TEXT
Lymphocytic Alveolitis, Bronchoalveolar Lavage Viral Load, and Outcome in Human Immunodeficiency Virus Infection
TWIGG et al.
Am. J. Respir. Crit. Care Med. 1999;159:1439-1444.
ABSTRACT
| FULL TEXT
Differential Tropism and Chemokine Receptor Expression of Human Immunodeficiency Virus Type 1 in Neonatal Monocytes, Monocyte-Derived Macrophages, and Placental Macrophages
Fear et al.
J. Virol. 1998;72:1334-1344.
ABSTRACT
| FULL TEXT
Differential Effects of Interleukin-13 on Cytomegalovirus and Human Immunodeficiency Virus Infection in Human Alveolar Macrophages
Hatch et al.
Blood 1997;89:3443-3450.
ABSTRACT
| FULL TEXT
Immunopathogenic Mechanisms in Human Immunodeficiency Virus (HIV) Infection
Fauci et al.
ANN INTERN MED 1991;114:678-693.
ABSTRACT
Regression of Oral Hairy Leukoplakia After Orally Administered Acyclovir Therapy
Resnick et al.
JAMA 1988;259:384-388.
ABSTRACT
Virus Isolation From and Identification of HTLV-III/LAV-Producing Cells in Brain Tissue From a Patient With AIDS
Gartner et al.
JAMA 1986;256:2365-2371.
ABSTRACT
HTLV-III/LAV and the Monocyte/Macrophage
Streicher and Joynt
JAMA 1986;256:2390-2391.
ABSTRACT
|
