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Isolation and Culture of the Bacterial Agent

LT Charles K. English, MSC; COL Douglas J. Wear, MC; Andrew M. Margileth, MD; LCDR Christopher R. Lissner, MSC; Gerald P. Walsh, PhD

JAMA. 1988;259(9):1347-1352.

Abstract
A gram-negative bacterium or its cell wall—defective variants were isolated from lymph nodes of ten patients with cat-scratch disease. Cultured bacteria were morphologically identical to vegetative and wall-defective forms seen in human tissues. Three of seven patients with recent cat-scratch disease had fourfold or greater rises in antibody titer against the cultured bacteria; the remaining four patients had maximum titers of 1:32 to 1:128. Rabbit antiserum to cultured bacilli reacted in immunoperoxidase stains with vegetative and wall-defective catscratch disease bacilli in lymph node, skin, or conjunctiva and with vegetative or wall-defective bacteria isolated from ten patients. Vegetative bacteria produced lesions in the skin of an armadillo identical to early lesions in human skin. Vegetative bacteria were recovered from the lesions in the armadillo.

(JAMA 1988;259:1347-1352)

Author Affiliations
USN; USA; USN

From the Department of Infectious and Parasitic Diseases Pathology, Armed Forces Institute of Pathology, Washington, DC (Drs English, Wear, Lissner, and Walsh); the Department of Pathology, Georgetown University School of Medicine, Washington, DC (Dr English); and the Department of Pediatrics, Uniformed Services University of the Health Sciences, F. Edward Hebert School of Medicine, Bethesda, Md (Dr Margileth).

Footnotes
The opinions or assertions contained herein are the private views of the authors and are not to be construed as official or as reflecting the views of the US Navy, the US Army, the US Department of Defense, the Uniformed Services University of the Health Sciences, or the Armed Forces Institute of Pathology.

Reprint requests to the Department of Infectious and Parasitic Diseases Pathology, CPS-M, Armed Forces Institute of Pathology, Washington, DC 20306-6000 (Dr Wear).

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