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Rapid Latex Agglutination Assay Using Recombinant Envelope Polypeptide for the Detection of Antibody to the HIV
Thomas C. Quinn, MD;
Charles H. Riggin, PhD;
Richard L. Kline, MS;
Henry Francis, MD;
Kabeya Mulanga, MS;
Michael G. Sension, MD;
Anthony S. Fauci, MD
JAMA. 1988;260(4):510-513.
Abstract
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Unscreened blood transfusions continue to be one of the major modes of transmission of the human immunodeficiency virus (HIV) in developing countries, such as in Central Africa, where 5% to 18% of blood donors are HIV seropositive. We evaluated a rapid latex agglutination assay using a novel recombinant envelope polypeptide of HIV for the detection of HIV antibodies among 2820 blood donors and clinical patients from diverse geographic regions, including on-site testing in Central Africa of 1600 blood donors. Overall, 29.2% of the serum samples were positive by Western blot assay. On a single determination, the latex agglutination slide test was found to be highly sensitive and specific compared with Western blot results in these populations with a relatively high prevalence of infection. Use of this assay will allow the immediate implementation of serologic screening for HIV in developing areas of the world, where standard screening procedures are impractical or not available, and in many other clinical settings, such as sexually transmitted diseases clinics and hospitals, where testing and counseling could be promptly implemented.
(JAMA 1988;260:510-513)
Author Affiliations
From the Laboratory of Immunoregulation, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Md (Drs Quinn, Francis, and Fauci); Cambridge Bio-Science Corp, Hopkinton, Mass (Dr Riggin); the Division of Infectious Diseases, The Johns Hopkins University School of Medicine, Baltimore (Drs Quinn and Sension and Mr Kline); and Project SIDA, Kinshasa, Zaire (Drs Francis and Sension and Mrs Mulanga).; Dr Riggin is an employee of Cambridge BioScience Corp. None of the other authors have any financial interest in Cambridge BioScience Corp. This work does not represent any specific endorsement by the US government of a commercial product.
Footnotes
Reprint requests to The Johns Hopkins Hospital, 600 N Wolfe St, Blalock 1111, Baltimore, MD 21205 (Dr Quinn).
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ABSTRACT
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