Frequent detection of genital herpes simplex virus DNA by polymerase chain reaction among pregnant women
R. W. Cone, A. C. Hobson, Z. Brown, R. Ashley, S. Berry, C. Winter and L. Corey
Department of Laboratory Medicine, University of Washington, Seattle.
OBJECTIVE--To investigate the prevalence and level of genital herpes
simplex virus (HSV) among women at delivery. DESIGN, PATIENTS, AND
SETTING--A prospective analysis of HSV by culture and by polymerase chain
reaction (PCR) of genital specimens and by HSV serologic studies in 100
asymptomatic women in labor; prospective analysis of HSV by PCR among 50
seronegative nonpregnant women at a student health center; and
retrospective analysis of genital specimens for HSV by PCR from 17 HSV
culture-positive women with uninfected neonates and from two HSV
culture-negative women with HSV-infected neonates. All pregnant women were
at a university hospital. MAIN OUTCOME MEASURES--Presence of HSV by culture
and levels of HSV by quantitative, type-specific PCR in cervical and vulvar
specimens; HSV serologic testing by Western blot. RESULTS--All of the 100
asymptomatic women in labor who were studied prospectively were HSV culture
negative. In nine HSV was recovered by PCR. Herpes simplex virus was
recovered by PCR in one of the 50 seronegative nonpregnant women; she soon
became seropositive. All 17 culture-positive women had HSV recovered by
PCR. High levels of HSV DNA were obtained by PCR from the two
culture-negative women with infected neonates. Among those from whom HSV
was recovered by PCR, HSV DNA levels were 250 times higher from
culture-positive samples than from culture-negative samples (11,571 genome
equivalents vs 46 genome equivalents; P < .001). CONCLUSIONS--The
frequency of infant exposure to HSV DNA-containing secretions from
HSV-seropositive mothers is about eight times higher than previously
reported using HSV culture methods. High maternal levels of HSV DNA may be
associated with an increased frequency of transmission of HSV to the
infant.
Genital Herpes Simplex Virus Infection and Perinatal Transmission of Human Immunodeficiency Virus
Chen et al.
Obstet Gynecol 2005;106:1341-1348.
ABSTRACT
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Genital Herpes Complicating Pregnancy
Brown et al.
Obstet Gynecol 2005;106:845-856.
ABSTRACT
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Poor Correlation Between Genital Lesions and Detection of Herpes Simplex Virus in Women in Labor
Gardella et al.
Obstet Gynecol 2005;106:268-274.
ABSTRACT
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Rapid Diagnosis of Herpes Simplex Virus Infection by a Loop-Mediated Isothermal Amplification Method
Enomoto et al.
J. Clin. Microbiol. 2005;43:951-955.
ABSTRACT
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Cesarean Delivery and Risk of Herpes Simplex Virus Infection--Reply
Brown et al.
JAMA 2003;289:2208-2209.
FULL TEXT
Differential detection of B virus and rhesus cytomegalovirus in rhesus macaques
Huff et al.
J. Gen. Virol. 2003;84:83-92.
ABSTRACT
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Seroprevalence of antibodies to herpes simplex virus types 1 and 2 among two sexually active female populations in Middlesbrough, England
Opaneye and Bashford
The Journal of the Royal Society for the Promotion of Health 2002;122:108-111.
ABSTRACT
The Application of a Plasmid DNA Encoding IFN-{{alpha}}1 Postinfection Enhances Cumulative Survival of Herpes Simplex Virus Type 2 Vaginally Infected Mice
Harle et al.
J. Immunol. 2001;166:1803-1812.
ABSTRACT
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Quantification of Fungal DNA by Using Fluorescence Resonance Energy Transfer and the Light Cycler System
Loeffler et al.
J. Clin. Microbiol. 2000;38:586-590.
ABSTRACT
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A Prospective Study of New Infections with Herpes Simplex Virus Type 1 and Type 2
Langenberg et al.
NEJM 1999;341:1432-1438.
ABSTRACT
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Development of a High-Throughput Quantitative Assay for Detecting Herpes Simplex Virus DNA in Clinical Samples
Ryncarz et al.
J. Clin. Microbiol. 1999;37:1941-1947.
ABSTRACT
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Detection and Direct Typing of Herpes Simplex Virus in Perianal Ulcers of Patients with AIDS by PCR
do Nascimento et al.
J. Clin. Microbiol. 1998;36:848-849.
ABSTRACT
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