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Thomas Thieme, PhD; Stephen Piacentini, MS; Sandra Davidson, RN; Karen Steingart, MD

JAMA. 1994;272(3):219-221.

Abstract
Objective.
—To determine if oral fluid samples can be used to reliably assess protective blood levels of antibodies to measles, mumps, and rubella.

Design.
—A comparison of matched serum and oral fluid samples from asymptomatic subjects in enzyme-linked immunosorbent assays for measles, mumps, and rubella antibodies. A longitudinal study compared matched serum and oral fluid samples from 11 subjects after measles-mumps-rubella vaccination.

Setting.
—Five US clinical sites with samples tested at the reporting laboratory.

Participants.
—A total of 157 asymptomatic subjects including 55 subjects younger than 18 years.

Main Outcome Measures and Results.
—The presence of antibodies in oral fluid specimens correlated with that in serum with the following levels of sensitivity and specificity: measles, 97% and 100%, respectively; mumps, 94% and 94%, respectively; rubella, 98% and 98%, respectively. Longitudinal studies of subjects after vac cination showed similar seroconversion profiles in oral fluid and serum samples.

Conclusion.
—Protective blood levels of antibodies to measles, mumps, and rubella can be assessed by means of an oral fluid sample with good reliability.

(JAMA. 1994;272:219-221)

Author Affiliations
From Epitope Inc, Beaverton, Ore (Dr Thieme, Mr Piacentini, and Ms Davidson), and Southwest Washington Health District, Vancouver (Dr Steingart). Dr Thieme, Mr Piacentini, and Ms Davidson own stock or have stock options in Epitope Inc, the manufacturer of the OraSure device.

Footnotes
Reprint requests to Epitope Inc, 8505 Creekside Place, Beaverton, OR 97005 (Dr Thieme).

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