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William E. Keene, PhD, MPH; Elizabeth Sazie, MD; Janet Kok, RN; Daniel H. Rice, MS; Dale D. Hancock, DVM, PhD; Vijay K. Balan, MS; Tong Zhao, MD; Michael P. Doyle, PhD

JAMA. 1997;277(15):1229-1231.

Abstract
Objective.
—To investigate a 1995 outbreak of Escherichia coli O157:H7 infections and to assess the safety of meat dehydration methods.

Design.
—Survey subsequent to routine surveillance report, environmental investigations, and laboratory experimentation.

Setting.
—Oregon community.

Participants.
—Members of an extended household and their social contacts with confirmed or presumptive E coli O157:H7 infections.

Results.
—A total of 6 confirmed and 5 presumptive cases were identified. Homemade venison jerky was implicated as the source of transmission. E coli 0157:H7 with the same distinctive, pulsed-field gel electrophoresis pattern seen in the case isolates was recovered from leftover jerky, uncooked meat from the same deer, a saw used to dismember the carcass, and fragments of the deer hide. In a subsequent survey, E coli O157:H7 was recovered from 3 (9%) of 32 deer fecal pellets collected in nearby forest land. In the laboratory, inoculated venison was dried at several time and temperature combinations, ranging up to 10 hours at 62.8°C. Viable organisms were recovered under all conditions tested.

Conclusions.
—Deer can be colonized by E coli O157:H7 and can be a source of human infections. Conditions necessary to ensure the safety of dried meat deserve further review. Game should be handled with the same caution indicated for commercially slaughtered meat.

Author Affiliations
From the Acute and Communicable Disease Program (Dr Keene) and the Oregon State Public Health Laboratory (Mr Balan), Oregon Health Division, Portland; Benton County Health Department, Corvallis, Ore (Dr Sazie and Ms Kok); Field Disease Investigation Unit, Washington State University, Pullman (Mr Rice and Dr Hancock); and the Center for Food Safety and Quality Enhancement, University of Georgia, Griffin (Drs Zhao and Doyle).

Footnotes
Corresponding author: William E. Keene, PhD, MPH, Oregon Health Division, 800 NE Oregon St, Suite 772, Portland, OR 97232 (e-mail: keene@ohsu.edu).

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