Effect of antioxidant vitamins on the transient impairment of endothelium-dependent brachial artery vasoactivity following a single high-fat meal

G. D. Plotnick, M. C. Corretti and R. A. Vogel
Department of Medicine, University of Maryland School of Medicine, Baltimore, USA. gplotnic@heart.ab.umd.edu

CONTEXT: Much has been written about the potential role of antioxidants in the prevention of atherosclerosis. OBJECTIVE: To assess the short-term effect of a single high-fat meal with and without pretreatment with antioxidant vitamins on endothelial function in healthy, normocholesterolemic subjects. DESIGN: Observer-blinded randomized trial. SETTING: University hospital. PARTICIPANTS: Twenty healthy, normocholesterolemic (total and low-density lipoprotein cholesterol <5.2 mmol/L and <3.4 mmol/L [<200 mg/dL and <130 mg/ dL], respectively), male (7) and female (13) hospital employee volunteers, aged 24 to 54 years. INTERVENTION: Three randomly administered breakfasts: (1) a high-fat meal (3766 J [900 calories], 50 g of fat); (2) a low-fat meal (3766 J [900 calories], 0 g of fat); and (3) a high-fat meal and pretreatment with oral administration of vitamins C (1 g) and E (800 IU) (high-fat meal with vitamins). A subgroup of 10 subjects also ate the low-fat meal with the same vitamin pretreatment (low-fat meal with vitamins). MAIN OUTCOME MEASURE: High-resolution ultrasound assessed flow-mediated (endothelium-dependent) brachial artery vasodilation measured as percent diameter change before and hourly for 6 hours following each meal. RESULTS: Flow-mediated vasodilation fell from a mean+/-SD of 20%+/-8% before to 12%+/-6%, 10%+/-6%, and 8%+/-9% at 2, 3, and 4 hours, respectively, after the high-fat meal (P<.001). No significant changes in flow-mediated vasodilation occurred after the low-fat meal, high-fat meal with vitamins, or low-fat meal with vitamins. The change in flow-mediated vasodilation after the low-fat and high-fat meals correlated inversely with the 2-hour postprandial change in triglyceride levels (r=-0.54; P<.001). CONCLUSION: A single high-fat meal transiently reduces endothelial function for up to 4 hours in healthy, normocholesterolemic subjects, probably through the accumulation of triglyceride-rich lipoproteins. This decrease is blocked by pretreatment with antioxidant vitamins C and E, suggesting an oxidative mechanism.

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ABSTRACT | FULL TEXT  

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ABSTRACT | FULL TEXT  

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ABSTRACT | FULL TEXT  

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ABSTRACT | FULL TEXT  

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ABSTRACT | FULL TEXT  

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ABSTRACT | FULL TEXT  

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ABSTRACT | FULL TEXT  

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Wilmink et al.
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ABSTRACT | FULL TEXT  

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ABSTRACT | FULL TEXT  

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Am. J. Clin. Nutr. 1999;69:1086-1107.
ABSTRACT | FULL TEXT  

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Herrington et al.
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ABSTRACT | FULL TEXT  

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Kugiyama et al.
J Am Coll Cardiol 1999;33:1512-1518.
ABSTRACT | FULL TEXT  

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FULL TEXT  

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Williams et al.
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ABSTRACT | FULL TEXT  

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Osada et al.
J. Appl. Physiol. 1999;86:709-719.
ABSTRACT | FULL TEXT  

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Haastrup et al.
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ABSTRACT | FULL TEXT  

Effect of Vitamins C and E on Vascular Reactivity
Kaufman et al.
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FULL TEXT  

Antioxidants and High-Fat Meals
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FULL TEXT  

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JWatch General 1997;1997:2-2.
FULL TEXT