German researchers have developed a sensitive new method to detect prions, the infectious proteins that cause neurodegenerative Creutzfeldt-Jakob disease (CJD) in humans and bovine spongiform encephalitis in cattle.
The technique, devised by researchers at the Max Planck Institute for Biophysical Chemistry and the University of Göttingen, both in Göttingen, Germany, uses fluorescent spectroscopy to detect prions in cerebrospinal fluid (CSF). Until now, the only way to arrive at a definite diagnosis of prion disease has been to locate accumulated prion protein in brain tissue during postmortem examinations.
By using specific antibody probes tagged with fluorescent dyes, the researchers were able to label the prion particles to appear as intensely bright targets when scanned in CSF with a laser beam. In a pilot study, the new technique demonstrated the presence of prions in the CSF of patients with CJD, but not in controls.
The researchers reported that the method . . . [Full Text of this Article]
