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Commentary by Gary Keeney, MD; Kevin Leslie, MD

JAMA. 2008;300(9):1074-1076.

	Since this article does not have an abstract, we have provided the first 150 words of the full text and any section headings.

ORIGINAL ARTICLE

A Method for the Rapid Preparation of Fresh Tissues for the Microscope

Louis B. Wilson, MD

JAMA. 1905;45:1737

While engaged in general pathologic work I shared the common distrust of frozen sections of fresh tissues for microscopic diagnosis. On taking charge recently of the laboratories of the Drs. Mayo, surgeons, I carefully tested the various methods hitherto published and found them either too slow for results while the patient waits under the anesthetic or else giving poorly differentiated cell detail. After considerable experimentation the following technic was discovered, and for the last six months it has given uniformly excellent preparations:

1. Bits of fresh tissue not more than 2 x 10 x 10 mm. are frozen in dextrin solution and cut in sections of from 10 to 15 microns thick.

2. The sections are removed from the knife with the . . . [Full Text of this Article]

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