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Pietro COCCHI, MD; Mario Silenzi, MD; Adriana Ravina, MD
University of Florence Florence, Italy

JAMA. 1978;240(8):737.

	Since this article does not have an abstract, we have provided the first 150 words of the full text PDF and any section headings.

To the Editor.—

Neutrophils of patients with Down's syndrome are known to have numerous abnormalities associated with diminished resistance to infection.^1,2 Several granulocyte functions have been measured in these patients: chemotaxis, phagocytosis, intracellular killing and metabolism; however, the survival of circulating neutrophils has not been investigated. The purpose of our study was to determine whether or not dead granulocytes are present in patients with trisomy 21 and in normal subjects.

A supravital staining, the eosin ion exclusion test of Hanks and Wallace³ as described elsewhere,⁴ was employed in this investigation. Loopspots of 1% aqueous eosin allowed to dry on a clean slide are rehydrated with 2-mm loops of heparinized fingertip or venous blood no more than 30 minutes after the removal. After two minutes at room temperature, the percentage of granulocytes containing eosin was determined by microscopic observation of droplets that have been fixed in 95% alcohol. . . . [Full Text PDF of this Article]

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